DEPARTMENT OF CELL BIOLOGY & MOLECULAR GENETICS

Overview

The origins of the department can be traced to the year 2010 when the Academy established a collaborative research center with the Genome Foundation of India under the mentorship of Late Padmashree Prof. Lalji Singh. The vision of this center was to elucidate the genomic landscape of the rural population in the Kolar area. This center then evolved into the Central Research Laboratory of the Academy so as to serve as a hub for interdisciplinary research between the Departments of the Academy. The Centre was elevated as a full-fledged statutory Department in the year 2013 for promoting academic programs in the subjects of Genetics and Molecular Biology.
We are mainly involved in three major activities viz., research, teaching, and clinical services.
We are equipped with state-of-the-art laboratory facilities for research in the area of Genomics, Molecular Biology, Cell Biology, Cytogenetics, and Phytochemistry. The department is housed in a dedicated centrally air-conditioned area of 8000 square feet.
We offer two-degree programs viz., M.Sc. degree in Molecular Biology and Human Genetics and a Ph.D. degree in Cell Biology and Human Genetics.

HOD’s note

I am pleased to share that we have made meaningful achievements in research matters. We had received extramural support from the Department of Health Research, Government of India for the conduct of a national training program in genetic techniques for medical college teachers. Academicians from several states have undergone training in this program. Our research activities have resulted in about 50 publications in UGC approved journals. At least half of these are in journals with an impact factor of over one. We have also filed for patents.

Vision

Creating knowledge and skills in human genetics, cell and molecular biology for a healthy humanity.

Mission

  • To impart skill based training to create a cadre of globally competent professionals in the area of human genetics, cell and molecular biology
  • To inculcate passion and nurture skills to carryout biomedical research among students, biomedical and healthcare professionals.
  • Objectives

  • To provide advanced infrastructure facilities to the students to become competent and responsible professionals in the area of human genetics, cell and molecular biology.
  • To design courses of innovative and interdisciplinary nature.
  • To promote facilities to carry out translational research by integrating basic and medical sciences.
  • To provide sophisticated molecular diagnostic facilities to understand the molecular basis of diseases.
  • Key Features

    The department is positioned as a hub for interdisciplinary research on the molecular basis of human diseases.

    Programs

    Master of Science (M. Sc.) in Molecular Biology and Human Genetics

    The program duration is 2 years (4 semesters) with 6 seats.
    The purpose of the program is to provide both knowledge and skills in molecular biology and human genetics. At the end of the program, the student will have an understanding of the genetic basis of biological functions and also the genetic basis of simple and complex diseases. Skill development involves both technical skills and also science communication. The student will gain the skills for evaluating chromosomal aberrations and genetic variations and also perform cell-based bioassays. The student will also be trained to conceive and evaluate a novel scientific idea that can expand the boundaries of science. Several of our students have published their research findings in UGC-approved journals.

    M.Sc. Cell Biology and Human Genetics Syllabus -Click here

    Eligibility Criteria

    A pass in bachelor’s degree in any branch of life sciences, medical laboratory technology, MBBS, BDS, BAMS, BHMS or other equivalent qualifications approved by SDUAHER with a minimum of second class securing at least 50% of the aggregative marks.

    Seat Matrix

    Number of seats: Six

    Selections

    Candidates are expected to appear for an entrance test to be conducted by the university department and thereafter an interview. Admission to the program will be based purely on merit obtained in the entrance examination. Application forms and other details will be made available by the office of the Registrar on payment of the prescribed application fee or can be downloaded from the website of the Academy www.sduu.ac.in. In case of such downloaded applications, the application should accompany a Demand Draft for the prescribed fee drawn in favor of The Registrar, SDUAHER, payable at Kolar. The applications should be complete in all respects and should accompany copies of relevant marks cards, degree certificates, caste certificate,s and date of birth certificate. The date, venue, and time of the entrance examination will be notified on the website to the applicants as soon as it is fixed.

    Reservation Policies (if any): As per the Academy norms

    Fee Structure: Academy’s decision

    Total Course Duration: Two years

    Admission Policy As Per Academy: Hostel Fees and Facilities

    Admission Procedure and details (if any) & others: Based on the entrance exam conducted by the Academy

    Ph. D. Program in Cell Biology and Molecular Genetics

    The Department provides a vibrant environment for pursuing Ph. D. degree, particularly in translational research. Our association with a medical institution provides opportunities for collaboration with clinicians and access to clinical material. Visit the human resources page for the list of potential guides and their research interest. Also, visit the research page for the list of Ph.D. students and their theses topics.

    Activities

    Orientation programs
    Camps
    Media & if any others
    National Web Conference on “Impact of environmental factors for plant and human genetics: Mitigation strategies”, jointly organized by University of Horticultural Sciences, Bagalkot, College of Horticulture, Kolar and Sri Devaraj Urs Academy of Higher Education and Research, Kolar.

    Facilities

    Sl. No. Name Photo
    1 DNA Sequencing Facility (Applied Biosystems, USA)
    2 Real Time PCR (Bio-Rad, USA)
    3 Automated Karyotyping System and Fluorescence Microscope (Carl Zeiss, Germany)
    4 FISH Hybridisation Chamber (ThermoBrite, Abbot, USA)
    5 Biosafety Cabinet (ClassII) (Ultra Biotech, India)
    6 Laminar Air Flow Bench (Esco, Singapore)
    7 Inverted microscope(Carl-Zeiss, Germany)
    8 CO2 incubator (Thermo Fisher Scientific, USA)
    9 Electro-Blotter (Bio-Rad, USA)
    10 UV-Vis Spectrophotometer (Perkin-Elmer, USA)
    11 Refrigerated Centrifuge (Thermo-Fisher Scientific, USA)
    12 ELISA Reader & Washer (Rayto, China)
    13 Extraction of Phytochemicals
    14 Deep Freezer (-80 °C)
    15 Deep Freezer (-20 °C)
    16 Milli Q water purification system
    17 Ice flaking machine
    18 PCR Thermal Cycler (Bio-Rad, USA)
    19 Gel-Doc (Bio-Rad, USA)

    Publications

    1. Gayathri BN, Sagayaraj A, Prabhakara S, Suresh TN, Shuaib M, Mohiyuddin SM. Papillary thyroid carcinoma in a 5-year-old child-case report. Indian J Surg Oncol. 2014 Dec;5(4):321-4.
    2. Vaigundan D, Kalmankar NV, Krishnappa J, Gowda NY, Kutty AV, Krishnaswamy PR. A novel mutation in the transglutaminase-1 gene in an autosomal recessive congenital ichthyosis patient. BioMed Research International. 2014;2014:706827.
    3. Pamidimukkala K, Anitha K, Rayalcheruvu U. Phytochemical investigation of Caralluma attenuata (Wight) roots. International Journal of Pharmacognosy and Phytochemical Research. 2015;7(5):1120-1124.
    4. Bose D, Krishnamurthy V, Venkatesh KS, Aiyaz M, Shetty M, Rao SN, Kutty AV. Molecular Delineation of Partial Trisomy 14q and Partial Trisomy 12p in a Patient with Dysmorphic Features, Heart Defect, and Developmental Delay. Cytogenetic and Genome Research. 2015;145(1):14-8.
    5. Vanilla S, Dayanand CD, Kotur PF, Kutty MA, Vegi PK. Evidence of Paternal N5, N10-Methylenetetrahydrofolate Reductase (MTHFR) C677T Gene Polymorphism in Couples with Recurrent Spontaneous Abortions (RSAs) in Kolar District- A South West of India. Journal of Clinical and Diagnostic Research. 2015 Feb;9(2):BC15-8.
    6. Malini K, Gudi NS, Kutty AV, Balakrishna S. Mutational Analysis of Exostosin 1 and 2 Genes in Multiple Osteochondroma. Indian Journal of Pediatrics. 2015 Jul;82(7):649-50.
    7. Pamidimukkala K, Anitha K, Rayalcheruvu U. Isolation and identification of steroid triterpenoids from the polar and non-polar fractions of Caralluma attenuata (Wight) roots. International Journal of Pharmacognosy and Phytochemical Research. 2016; 8(6); 912-929.
    8. Hanna M, Brophy PD, Giannone PJ, Joshi MS, Bauer JA, Ramachandra Rao S. Early urinary biomarkers of acute kidney injury in preterm infants. Pediatric Research. 2016 Aug;80(2):218-23.
    9. Chu LL, Katzberg RW, Solomon R, Southard J, Evans SJ, Li CS, McDonald JS, Payne C, Boone JM, RamachandraRao SP. Clinical Significance of Persistent Global and Focal Computed Tomography Nephrograms After Cardiac Catheterization and Their Relationships to Urinary Biomarkers of Kidney Damage and Procedural Factors: Pilot Study. Investigative Radiology. 2016 Dec;51(12):797-803.
    10. Awdishu L, Nievergelt CM, Davenport A, Murray PT, Macedo E, Cerda J, Chakaravarthi R, Ramachandra Rao SP, Holden A, Goldstein SL, Mehta RL. Rationale and Design of the Genetic Contribution to Drug-Induced Renal Injury (DIRECT) Study. Kidney International Reports. 2016 Aug 24;1(4):288-298.
    11. Vijaykumar BR, Lekshmi SU, Sai Kant R, Vaigundan D, Mahadevan A, Rajendran C, Shankar SK, Jayshree RS. Genetic characterization of Toxoplasma gondii from autopsy proven cases of AIDS-associated cerebral toxoplasmosis in South India. Infection, Genetics and Evolution. 2016 Apr;39:106-112.
    12. Dayanand K, Girish ST. Bio-diesel production from residual sunflower oil by trans-esterification (Acidic, alkaline enzymatic) and analysis of some of its Physico-chemical parameters. Annual Research and Review in Biology. 2017;20(5).
    13. Patras KA, Coady A, Olson J, Ali SR, Ramachandra Rao SP, Kumar S, Varki A, Nizet V. Tamm-Horsfall glycoprotein engages human Siglec-9 to modulate neutrophil activation in the urinary tract. Immunology and Cell Biology. 2017 Nov;95(10):960-965.
    14. Nelaturi PD, Sriramaia NH, Nagaraj S, Kotakadi VS, Veeran Moideen Kutty AV, Pamidimukkala K. An in-vitro cytotoxic and genotoxic properties of Allamanda Cathartica L. Latex Green NPs on human peripheral blood mononuclear cells. Nano Biomedicine and Engineering. 2017;9(4):314-323.
    15. Chengalvala K, Kotur P, Shetty M, Kumar P, TV J, Sivaraj N, Balakrishna S. Association of maternal angiotensinogen gene M235T polymorphism with preeclampsia in South India: A tertiary care hospital-based case-control study. Meta Gene. 2017; 11:108-110.
    16. Rajesh D, Chowdappa C, Gurumurthy R, Moideen Kutty AV, Balakrishna S. Profile of tumor necrosis factor-alpha -308 G/A gene polymorphism in psoriatic patients in Karnataka, India. Journal of Clinical and Diagnostic Research. 2017;11(2):1-4.
    17. Rajesh D, Gurumurthy R, Kutty AV, Balakrishna S. Tumor necrosis factor-alpha gene promoter 238G/A polymorphism increases the risk of psoriasis Vulgaris in Indian patients. International Journal of Dermatology. 2017 Mar;56(3):307-311.
    18. Rajesh D, Mohiyuddin SMA, Kutty AVM, Balakrishna S. Prevalence of human papillomavirus in oral squamous cell carcinoma: A rural teaching hospital-based cross-sectional study. Indian Journal of Cancer. 2017 Jul-Sep;54(3):498-501.
    19. Bose D, D V, Shetty M, J K, Kutty AVM. Identification of intronic-splice site mutations in GATA4 gene in Indian patients with congenital heart disease. Mutation Research. 2017 Oct;803-805:26-34.
    20. Kavya D., Girish ST. Evaluation of biodiesel obtained from Pongamia and residual palm oil and its Physico-chemical parameters. Asian Journal of Microbiology, Biotechnology, and Environmental Sciences. 2018;20(3):911-917.
    21. Srinivasan P, Mary I, Nandini H, Kutty AVM, Pamidimukkala K. A pilot study on sun protection factor of plant extracts: An observational study. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11(4):67-71.
    22. Ramasarma T, Vaigundan D. Alternative pathway linked by hydrogen bonds connects heme-Fe of cytochrome c with subunit II-CuA of cytochrome a. Biochemical and Biophysical Research Communications. 2018 Oct 28;505(2):445-447.
    23. Nagarajappa C, Rangappa SS, Suryanarayana R, Balakrishna S. Urinary congophilia in preeclampsia: Experience from a rural tertiary-care hospital in India. Pregnancy Hypertension. 2018 Jul;13:83-86.
    24. Rajesh D, Balakrishna S, Azeem Mohiyuddin SM, Suresh TN, Moideen Kutty AV. GSTP1 c.341C>T gene polymorphism increases the risk of oral squamous cell carcinoma. Mutation Research – Genetic Toxicology and Environmental Mutagenesis.2018 Jul;831:45-49.
    25. Rani U, Praveen Kumar KS, Munisamaiah M, Rajesh D, Balakrishna S. Atrial fibrillation associated genetic variation near PITX2 gene increases the risk of preeclampsia. Pregnancy Hypertension. 2018 Jul;13:214-217.
    26. Rajesh D, Azeem Mohiyuddin SM, Balakrishna S, Kutty AVM. Nicotinic acetylcholine receptor gene polymorphism is not associated with tobacco-related oral squamous cell carcinoma. Indian Journal of Cancer. 2018 Oct-Dec;55(4):399-403.
    27. Pamidimukkala K, Inala MSR. Loranthus lonfilorous Desr: A wise host range parasite harbored on a few new hosts on Sri Devaraj Urs Academy of Higher Education and Research campus and phytochemical comparison of host and parasite. Ecology, Environment and Conservation Paper. 2019, 25:1756-1765
    28. Gandham R, Sumathi ME, Dayanand CD, Sheela SR, Kiranmayee P. Neutrophil and platelet to lymphocyte ratio in prevailing the oxidative stress and its relation with the endothelial dysfunction in preeclampsia. Journal of Krishna Institute of Medical Sciences University. 2019; 8 (4) : 89-97.
    29. Nagarajappa C, Rangappa SS, Balakrishna S. Misfolding linked mutations of SERPINA1 gene are uncommon in preeclampsia. Archives of Medicine and Health Sciences.. 2019;7:177-180.
    30. Ramasarma T, Vaigundan D. Pathways of electron transfer and proton translocation in the action of superoxide dismutase dimer. Biochem Biophys Res Commun. 2019 Jun 30;514(3):772-776.
    31. Ramasarma T, Vaigundan D. Pathways linked by hydrogen bonds with redox-dependent breaks implicated in electron. Indian Journal of Biochemistry and Biophysics. 2019;56(1):46-52. (IF: 0.38).
    32. Ramasarma T, Vaigundan D. Connecting CuA with metal centers of heme a, heme a 3, CuB, and Zn by pathways with hydrogen bond as the bridging element in cytochrome c oxidase. Biochemical and Biophysical Research Communications. 2019 Mar 5;510(2):261-265. (IF: 2.56).
    33. Ramasarma T, Vaigundan D. Hydrogen bond-linked pathways of peptide units and polar groups of amino acid residues suitable for electron transfer in cytochrome c proteins. Molecular and Cellular Biochemistry. 2019 Mar;453(1-2):197-203.
    34. Rajesh D, Nagraj S, Kumar PKS, Kutty AVM, Balakrishna S. Evaluation of HCP5 and CCR5 gene polymorphisms in Indian psoriatic patients. Indian Journal of Dermatology, 2019; 64, 182-186.
    35. Zainabadi K, Dhayabaran V, Moideen K, Krishnaswamy P. An efficient and cost-effective method for purification of small-sized DNAs and RNAs from human urine. PLoS One. 2019 Feb 5;14(2):e0210813.
    36. Nagarajappa C, Sheela SR, Suryanarayana R, Balakrishna S. Urinary protein carbonyl levels and its correlation with protein misfolding in preeclampsia. Hypertension in Pregnancy. 2019 Mar 26;38(2):124-128.
    37. Kumar KSP, Madhuri A, Munisamaiah M, Balakrishna S. Novel association of SNP rs479200 in EGLN1 gene with a predisposition to preeclampsia. Gene, 2019,705,1-4.
    38. Rajesh D, Balakrishna S, Azeem Mohiyuddin SM, Suryanarayana R, Kutty AVM. Novel association of oral squamous cell carcinoma with GSTP1 Arg187Trp gene polymorphism. Journal of Cellular Biochemistry. 2019 Apr;120(4):5906-5912.
    39. Kiranmayee P, Prabhudas N, Nandini HS, Kutty AVM. Comparative analysis of transition and post-transition metal-mediated Allamanda cathartica L latex nanoparticles on human peripheral blood mononuclear cells. Advances in Natural Sciences: Nanoscience and Nanotechnology. 2020; 11(1): 015016
    40. Dhayabaran V, Chidambaram D, Krishnaswamy PR. Identification of compounds for improved growth of Leptospira in culture and isolation. Diagn Microbiol Infect Dis. 2020 Jan;96(1):114923.
    41. Veeregowda SH, Krishnaswamy B, Balakrishna S. Effect of Vitamin K epoxide reductase complex 1 polymorphism on warfarin dose requirement among patients in tertiary care hospital. Int J App Basic Med Res 2020;10:97-101.
    42. Shetty M, Balakrishna S, Hegde S. Inadequate pre-conception counseling is a major challenge for antenatal management of β-thalassemia: Experience from a referral center in India. Indian Journal of Hematology and Blood Transfusion. 2020 Apr;36(2):414-416.
    43. Francis I, Arcot M, Mohiyuddin ASM, Balakrishna S. Hypoxia responsiveness linked variant in EGLN1 gene is enriched in oral cancer patients. Archives of Oral Biology. 2020;116: 104767.
    44. Changalvala K, Prabhavathi K, Venkateshu KV, Sheela SR, Kalyani R, Raghuveer CV, Kiranmayee P. Fluoride as a risk factor in pre-eclampsia. Fluoride. 2020;53(3 Pt 1):402-424
    45. Inala MSR, Karthikkeyan G, Pinto SM, Pamidimukkala K. Biological Activity of ‘3, 3′, 4′, 5, 7-Pentahydroxyflavone Isolated from Green Leafy Vegetables of Karnataka on three Bacterial Strains. Biomed Pharmacol J. 2020;13(2):849-863.
    46. Rajeev Gandham, Dayanand CD, Sheela SR, Kiranmayee P. Impact of Oxidative Stress on Maternal Serum Apelin 13 and Endothelial Nitric Oxide Synthase in Preeclampsia. Biomed Pharmacol J. 2020; 13 (4):2041-2048.
    47. Raavi, V., Perumal, V., F.D. Paul, S. Potential application of γ-H2AX as a biodosimetry tool for radiation triage. Mutation Research – Reviews in Mutation Research, 2021, 787, 108350
    48. Gandham R, Dayanand CD, Sheela SR, Kiranmayee P. Maternal serum Apeln 13 and APLN gene promoter variant -1860T > C in preeclampsia. J Matern Fetal Neonatal Med. 2021 Jan 17:1-9.
    49. Siddulakshmi Prasanna, Prasannakumar MK, Mahesh HB, Gopal Venkatesh Babu, Kiranmayee P, Puneeth ME, Karthik SN, Pramesh D. Diversity and biopotential of Bacillus velezensis strains A6 and P42 against rice blast and bacterial light of pomegranate. Arch. Microbiol. May 2021. 10.1007/s00203-021-02400-9

    Contact Details

    Phone : 08152-210604, ext: 402
    Email : genomelab@sduu.ac.in

    Faculty

    Dr. B. Sharath

    Dr. P. Kiranmayee

    Dr. Venkateswarlu Raavi